Localization of Neurophysin within Organelles Associated with Protein Synthesis and Packaging in the Hypothalamo-neurohypophysial System: An Immunocytochemical Study

Electron microscopic immunocytochemical localization of neurophysin in the hypothalamo-neurohypophysial system of mice has been studied by using the preembedding staining approach to the unlabeled antibody-enzyme technique of Sternberger. In supraoptic cell bodies, peroxidase-antiperoxidase reaction...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1979-11, Vol.76 (11), p.5999-6003
Main Authors: Broadwell, Richard D., Oliver, Constance, Brightman, Milton W.
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Antiserum
Lysosomes
Neurons
Neurophysins
Organelles
Packaging
Reaction products
Secretory vesicles
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Electron microscopic immunocytochemical localization of neurophysin in the hypothalamo-neurohypophysial system of mice has been studied by using the preembedding staining approach to the unlabeled antibody-enzyme technique of Sternberger. In supraoptic cell bodies, peroxidase-antiperoxidase reaction product was localized within cisternae of the nuclear envelope, rough endoplasmic reticulum, and Golgi saccules but not in GERL (Golgi-associated smooth endoplasmic reticulum from which lysosomes arise). Reaction product was also present in secondary lysosomes. Secretory granules in supraoptic perikarya and posterior pituitary Herring bodies were likewise immunoreactive. These findings with the unlabeled antibody-enzyme technique provide conclusive evidence for the localization of an antigen within cellular organelles associated with protein synthesis and packaging.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.11.5999