Spontaneous Action Potentials in Isolated Guinea-Pig Cerebellar Slices: Effects of Amino Acids and Conditions Affecting Sodium and Water Uptake

The effects of incubation conditions on the frequency of spontaneous action potentials exhibited by guinea-pig cerebellar slices, and recorded with an extracellular microelectrode, have been investigated. Various incubation conditions that lead to tetrodotoxin-sensitive uptakes of water and of sodiu...

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Bibliographic Details
Published in:Proceedings of the Royal Society of London. Series B, Biological sciences Biological sciences, 1973-08, Vol.184 (1074), p.83-90
Main Authors: Okamoto, K., Quastel, Juda Hirsch
Format: Article
Language:English
Subjects:
Action potentials
Action Potentials - drug effects
Amino acids
Amino Acids - pharmacology
Animals
Anoxia
Cerebellar cortex
Cerebellum
Cerebellum - drug effects
Cerebellum - metabolism
Cerebellum - physiology
Cerebral cortex
In Vitro Techniques
Purkinje cells
Rats
Sodium
Sodium - metabolism
Tetrodotoxin
Tetrodotoxin - pharmacology
Water - metabolism
Water uptake
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Summary:The effects of incubation conditions on the frequency of spontaneous action potentials exhibited by guinea-pig cerebellar slices, and recorded with an extracellular microelectrode, have been investigated. Various incubation conditions that lead to tetrodotoxin-sensitive uptakes of water and of sodium ions by the incubated cerebellar slices lead to enhanced frequencies of the spontaneous action potentials, e. g. the presence of protoveratrine or of ouabain, the absence of glucose or the onset of anoxia. The frequency of the spikes is also enhanced by acetylcholine (in presence of neostigmine) or by the presence of excitatory amino acids, such as L-glutamate, D-glutamate or L-aspartate. It is suppressed by tetrodotoxin, or by the inhibitory amino acids, e. g. γ-aminobutyrate, glycine or taurine, or by ammonium ions or by pentobarbital. It is concluded that guinea-pig cerebellar slices, incubated under specified conditions, may provide a suitable means for quantitative correlation of neurochemical data with data obtained by electrophysiological techniques in tissue incubated under similar conditions and also for quantitative assessment of the effects of amino acids on cerebellar electrical activity.
ISSN:0962-8452
0080-4649
0950-1193
1471-2954
2053-9193
DOI:10.1098/rspb.1973.0032