Analysis of Rat Heart in vivo by Phosphorus Nuclear Magnetic Resonance

High-resolution31P nuclear magnetic resonance spectra at 73.83 MHz are reported for rat heart in vivo. In live rats, it was possible to observe the cardiac content of ATP, phosphocreatine, and Pi. Only a small amount of whole-blood 2,3-diphosphoglycerate was observed in the spectra, precluding the p...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1980-01, Vol.77 (1), p.299-302
Main Authors: Grove, Thomas H., Joseph J. H. Ackerman, Radda, George K., Bore, Peter J.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Biochemistry
Blood
Cardiac arrest
Chemical equilibrium
Energy metabolism
Fourier transformations
Heart
Magnetic Resonance Spectroscopy - methods
Male
Myocardium - analysis
Myocardium - metabolism
Nuclear magnetic resonance
Perfusion
Phosphates
Phosphocreatine - metabolism
Phosphorus
Rats
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Summary:High-resolution31P nuclear magnetic resonance spectra at 73.83 MHz are reported for rat heart in vivo. In live rats, it was possible to observe the cardiac content of ATP, phosphocreatine, and Pi. Only a small amount of whole-blood 2,3-diphosphoglycerate was observed in the spectra, precluding the possibility that blood phosphate compounds were masking the spectra of cardiac phosphate compounds. The31P nuclear magnetic resonance spectra of in vivo and perfused rat hearts were similar and support the utilization of the perfused rat heart as a model system for studying high-energy phosphate metabolism of the heart in vivo. The dynamic flux of high-energy phosphate compounds was investigated by subjecting the rat to respiratory arrest. In this experiment, the heart followed the classic metabolic pattern known to occur during cardiac arrest; phosphocreatine and then ATP decreased in concentration while Piincreased in concentration. The31P nuclear magnetic resonance analysis of rat heart in vivo is demonstrated to be a practical and feasible method for studying cardiac high-energy phosphate metabolism.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.1.299