THE USE OF BIOLUMINESCENCE OF BEAUVERICIN TOXICITY TO WHEAT

The purpose of our work was to assess whether BEA can affect the root and leaf cells of wheat cultivar (cv.) "Arina" seedlings, using cytotoxicity assay and fluorescent microscopy. Leaf primordial, situated at the base, and the tips of treated leaves were affected by BEA when assayed in me...

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Bibliographic Details
Published in:Cereal research communications 2008-01, Vol.36, p.393-394
Main Authors: ŠROBÁROVÁ, Antónia, OVEČKA, Miroslav, OVEČKOVÁ, Ingrid
Format: Article
Language:English
Subjects:
Apoptosis
Cell death
Fusarium
Luminescence
Microscopy
Mycotoxins
Plant cells
Plants
Seedlings
Viability
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Summary:The purpose of our work was to assess whether BEA can affect the root and leaf cells of wheat cultivar (cv.) "Arina" seedlings, using cytotoxicity assay and fluorescent microscopy. Leaf primordial, situated at the base, and the tips of treated leaves were affected by BEA when assayed in medium for cell viability measured by luminescent equipment. BEA-treated plant cells secrete adenosine triphosphate (ATP) to the extracellular matrix and invoke more luminescence by luciferase than the non-treated seedlings. Our results were confirmed by fluorescence microscopy and by confocal microscopy.
ISSN:0133-3720
1788-9170