Electron Microscope Study of Duck Globin mRNA Precursor Crosslinked in situ

Double-stranded RNA segments present in duck globin pre-mRNA were crosslinked in situ with aminomethyltrioxalen and UV light. The secondary structure of the crosslinked pre-mRNA was then studied by electron-microscopic analysis of pre-mRNA· cDNA hybrids. The data suggest that duck globin pre-mRNAs c...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1980-08, Vol.77 (8), p.4683-4687
Main Authors: Reymond, Christophe, Agliano, Annamaria, Spohr, Georges
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Complementary DNA
Cross-Linking Reagents
Ducks
Ducks - genetics
Globins - genetics
Hybrid structures
Hybridity
Hydrogen Bonding
Introns
Messenger RNA
Microscopy, Electron
Molecular Weight
Molecules
Nucleic Acid Hybridization
Nucleic Acid Precursors - genetics
RNA
RNA precursors
RNA, Heterogeneous Nuclear - genetics
RNA, Messenger - genetics
Trioxsalen
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Summary:Double-stranded RNA segments present in duck globin pre-mRNA were crosslinked in situ with aminomethyltrioxalen and UV light. The secondary structure of the crosslinked pre-mRNA was then studied by electron-microscopic analysis of pre-mRNA· cDNA hybrids. The data suggest that duck globin pre-mRNAs contain intervening sequences that are excised stepwise. Excision and subsequent ligation appears to occur on precursor molecules that are stabilized by base-paired regions.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.8.4683