Human SGBS Cells – a Unique Tool for Studies of Human Fat Cell Biology

The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimite...

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Bibliographic Details
Published in:Obesity facts 2008-08, Vol.1 (4), p.184-189
Main Authors: Fischer-Posovszky, Pamela, Newell, Felicity S., Wabitsch, Martin, Tornqvist, Hans E.
Format: Article
Language:English
Subjects:
Abnormalities, Multiple - physiopathology
Adipocyte
Adipocytes - cytology
Adipocytes - physiology
Adipose Tissue - cytology
Adipose Tissue - physiology
Animals
Cell Line
Fat cell
Health Sciences
Humans
Hälsovetenskap
Medical and Health Sciences
Medicin och hälsovetenskap
Nutrition and Dietetics
Näringslära
Obesity - physiopathology
Review
Review Article
SGBS
Syndrome
Online Access:Get full text
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Summary:The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimited source due to their ability to proliferate for up to 50 generations with retained capacity for adipogenic differentiation. So far, the cells have been used for a number of studies on adipose differentiation, adipocyte glucose uptake, lipolysis, apoptosis, regulation of expression of adipokines, and protein translocation. The cells are efficiently differentiated in the presence of PPARγagonists and in the absence of serum and albumin. SGBS adipocytes respond to insulin stimulation by increasing glucose uptake several-fold (EC50 approximately 100 pmol/l), and by very effectively inhibiting (IC50 approximately 10 pmol/l) catecholamine-stimulated lipolysis.
ISSN:1662-4025
1662-4033
1662-4033
DOI:10.1159/000145784