Isolation and Properties of Human α-Fetoprotein from HepG2 Cell Cultures

A relatively rapid 3-step fractionation method has been developed for the isolation of human α-fetoprotein from culture fluids of HepG2 cells applicable to large volumes. The protein exists as a complex with lipids or lipoproteins but an ethanol precipitation step is effective in separating it. Yiel...

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Bibliographic Details
Published in:Tumor biology 2000-09, Vol.21 (5), p.267-277
Main Authors: Deutsch, Harold F., Taniguchi, Naoyuki, Evenson, Merle A.
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - isolation & purification
Biological and medical sciences
Host-tumor relations. Immunology. Biological markers
Humans
Isoelectric Focusing
Liver Neoplasms - chemistry
Medical sciences
Molecular Weight
Original Paper
Tumor Cells, Cultured
Tumors
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Summary:A relatively rapid 3-step fractionation method has been developed for the isolation of human α-fetoprotein from culture fluids of HepG2 cells applicable to large volumes. The protein exists as a complex with lipids or lipoproteins but an ethanol precipitation step is effective in separating it. Yields of 50–60% can be obtained from culture fluid containing 30–40 μg/ml. A minor fraction that appears to be a proteolytic product of the AFP is present in the final product.
ISSN:1010-4283
1423-0380
DOI:10.1159/000030132