A New Technique to Induce Experimental Myointimal Hyperplasia

Background: Arterial myointimal hyperplasia (MIH) has a significant impact on the long-term outcomes of vascular procedures such as bypass surgery and angioplasty. In this study, we describe a new and innovative technique to induce MIH using a dental flossing cachet in Wistar rats. Methods: The inti...

Full description

Saved in:
Bibliographic Details
Published in:Medical principles and practice 2018-01, Vol.27 (5), p.415-419
Main Authors: Asfar, Sami, Shuaib, Ali, Al-Otaibi, Fatemah, Asfar, Sora S., Kilarkaje, Narayana
Format: Article
Language:English
Subjects:
Angioplasty
Animals
Automation
Carotid arteries
Carotid Arteries - physiopathology
Carotid Arteries - surgery
Catheters
Dental Devices, Home Care
Disease Models, Animal
Hyperplasia
Medicine
Original Paper
Pathogenesis
Rats
Rats, Wistar
Smooth muscle
Stents
Tunica Intima - physiopathology
Tunica Intima - surgery
Veins & arteries
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Arterial myointimal hyperplasia (MIH) has a significant impact on the long-term outcomes of vascular procedures such as bypass surgery and angioplasty. In this study, we describe a new and innovative technique to induce MIH using a dental flossing cachet in Wistar rats. Methods: The intimal damage in the common carotid artery was induced by inserting the tip of the dental flossing cachet through the external carotid artery into the common carotid artery and turning it on for 3 rounds of 20 s each (n = 10). After 2 weeks, the rats were anesthetized and the common carotid arteries of the experimental side and the contralateral side (control) were harvested and preserved for histopathological studies. Results: The experimental carotid arteries showed significant intimal proliferation and thickening compared to the controls. The intima/media ratio of the experimental and normal (control) common carotid arteries were 1.274 ± 0.162 and 0.089 ± 0.023 (mean ± SEM), respectively (p < 0.001). Conclusion: This technique is simple, inexpensive, and highly reproducible and it induces sufficient MIH to study this phenomenon in animal models.
ISSN:1011-7571
1423-0151
DOI:10.1159/000492575