A New Technique to Induce Experimental Myointimal Hyperplasia

• Published in: Medical principles and practice 2018-01, Vol.27 (5), p.415-419
• Main Authors: Asfar, Sami, Shuaib, Ali, Al-Otaibi, Fatemah, Asfar, Sora S., Kilarkaje, Narayana
• Format: Article
• Language: English
• Subjects: Angioplasty; Animals; Automation; Carotid arteries; Carotid Arteries - physiopathology; Carotid Arteries - surgery; Catheters; Dental Devices, Home Care; Disease Models, Animal; Hyperplasia; Medicine; Original Paper; Pathogenesis; Rats; Rats, Wistar; Smooth muscle; Stents; Tunica Intima - physiopathology; Tunica Intima - surgery; Veins & arteries
• ISSN: 1011-7571; 1423-0151
• DOI: 10.1159/000492575

Background: Arterial myointimal hyperplasia (MIH) has a significant impact on the long-term outcomes of vascular procedures such as bypass surgery and angioplasty. In this study, we describe a new and innovative technique to induce MIH using a dental flossing cachet in Wistar rats. Methods: The intimal damage in the common carotid artery was induced by inserting the tip of the dental flossing cachet through the external carotid artery into the common carotid artery and turning it on for 3 rounds of 20 s each (n = 10). After 2 weeks, the rats were anesthetized and the common carotid arteries of the experimental side and the contralateral side (control) were harvested and preserved for histopathological studies. Results: The experimental carotid arteries showed significant intimal proliferation and thickening compared to the controls. The intima/media ratio of the experimental and normal (control) common carotid arteries were 1.274 ± 0.162 and 0.089 ± 0.023 (mean ± SEM), respectively (p < 0.001). Conclusion: This technique is simple, inexpensive, and highly reproducible and it induces sufficient MIH to study this phenomenon in animal models.