Effects of Octreotide Pretreatment in Experimental Acute Pancreatitis

• Published in: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2003-01, Vol.3 (2), p.164-168
• Main Authors: Salem, Marcelo Z., J. Cunha, Eduardo M., Coelho, Ana M., Sampietri, Sandra N., Machado, Marcel C.C., Penteado, Sonia, Abdo, Emilio E.
• Format: Article
• Language: English
• Subjects: Acute Disease; Acute pancreatitis; Amylases - metabolism; Animals; Ascitic Fluid - metabolism; Ceruletide - administration & dosage; Ceruletide - therapeutic use; Disease Models, Animal; Drug Therapy, Combination; Gastrointestinal Agents - administration & dosage; Gastrointestinal Agents - therapeutic use; Injections, Intravenous; Male; Octreotide; Octreotide - administration & dosage; Octreotide - therapeutic use; Original Paper; Pancreas - drug effects; Pancreas - enzymology; Pancreatic Elastase - metabolism; Pancreatic enzymes; Pancreatitis - drug therapy; Pancreatitis - enzymology; Pancreatitis - pathology; Pulmonary Edema - chemically induced; Pulmonary Edema - drug therapy; Pulmonary Edema - pathology; Pulmonary lesions; Rats; Rats, Wistar; Taurocholic Acid - pharmacology; Trypsin - metabolism
• ISSN: 1424-3903; 1424-3911
• DOI: 10.1159/000070086

Background: Severity of systemic lesions and mortality of experimental acute pancreatitis (AP) are reduced after pancreatic enzyme content reduction induced by cerulein administration. Octreotide has been used both pro- phylatically and therapeutically in AP. The possible effects of octreotide on pancreatic enzyme content and its influence on pulmonary lesions of experimental AP were assessed in this study. Methods: Wistar male rats were divided in two branches: Branch I - Animals divided into three groups: Group Sa (n = 10) intravenous saline infusion; Group Ce (n = 10) intravenous cerulein infusion, (0.133 ig/kg -1· h -1 ) and Group Oc (n = 10) SC octreotide (10 ig/kg -1 ). Trypsin, elastase and amylase pancreatic contents as well as serum amylase were determined thereafter in all three groups; Branch II - Rats treated as in branch I, were submitted to sodium taurocholate AP (Groups Sa+AP, Ce+AP and Oc+AP). Two hours thereafter amylase and TAP assays were performed in serum, ascites and pancreatic tissue in eight animals of each group. Pulmonary histology was studied by morphometry 24 h after AP in the remaining animals.Results: Increased serum amylase and pancreatic enzyme contents were observed in octreotide-treated animals when compared to animals receiving saline or cerulein. After AP increases of serum and ascitic fluid amylase and of pancreatic TAP were observed in octreotide pre-treated animals when compared to saline and cerulein groups. Pulmonary interstitial and alveolar edema after AP was significantly increased in rats receiving octreotide as compared to the cerulein group.Conclusion: Octreotide administration acutely increases the enzymatic content of the pancreas and thus may have a potential deleterious influence in the evolution of AP.