Notch1 Has an Important Role in β-Cell Mass Determination and Development of Diabetes

Background: Notch signaling pathway plays an important role in regulating pancreatic endocrine and exocrine cell fate during pancreas development. Notch signaling is also expressed in adult pancreas. There are few studies on the effect of Notch on adult pancreas. Here, we investigated the role of No...

Full description

Saved in:
Bibliographic Details
Published in:Diabetes & metabolism journal 2021-01, Vol.45 (1), p.86
Main Authors: Young Sil Eom, A-ryeong Gwon, Kyung Min Kwak, Jin-young Youn, Heekyoung Park, Kwang-won Kim, Byung-joon Kim
Format: Article
Language:Korean
Subjects:
Diabetes mellitus
Glucose
Insulin secretion
Insulin-secreting cells
Notch
Receptors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Notch signaling pathway plays an important role in regulating pancreatic endocrine and exocrine cell fate during pancreas development. Notch signaling is also expressed in adult pancreas. There are few studies on the effect of Notch on adult pancreas. Here, we investigated the role of Notch in islet mass and glucose homeostasis in adult pancreas using Notch1 antisense transgenic (NAS). Methods: Western blot analysis was performed for the liver of 8-week-old male NAS mice. We also conducted an intraperitoneal glucose tolerance test (IPGTT) and intraperitoneal insulin tolerance test in 8-week-old male NAS mice and male C57BL/6 mice (control). Morphologic observation of pancreatic islet and β-cell was conducted in two groups. Insulin secretion capacity in islets was measured by glucose-stimulated insulin secretion (GSIS) and perifusion. Results: NAS mice showed higher glucose levels and lower insulin secretion in IPGTT than the control mice. There was no significant difference in insulin resistance. Total islet and β-cell masses were decreased in NAS mice. The number of large islets (≥250 μm) decreased while that of small islets (
ISSN:2233-6079