A modified hydrostatic microfluidic pumpless device for in vitro murine ovarian tissue culture as research model for fertility preservation

This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device, MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice wer...

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Bibliographic Details
Published in:Obstetrics & gynecology science 2022-07, Vol.65 (4), p.376
Main Authors: Paweena Thuwanut, Alongkorn Pimpin, Fueangrat Thatsanabunjong, Sayamon Srisuwatanasagul, Wisan Sereepapong, Porntip Sirayapiwat
Format: Article
Language:Korean
Subjects:
Crofluidics
Fertility preservation
In vitro technique
Ovarian follicle
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Summary:This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device, MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice were divided into three groups: 1) fresh control, 2) conventional culture system (control), and 3) non-conventional system with MHPD. Ovarian tissues were cultured for 96 hours and evaluated for follicle morphology, developmental stage, intact follicle density, and relative gene expression levels (proliferating cell nuclear antigen, insulin like growth factor 1, BAX, and Bcl-2). Our major data demonstrated that the mean percentage of primary follicle development was increased by the MHPD (P
ISSN:2287-8572