NMR Study of Effects of $MgCl_2$ on the Structural and Dynamical Properties of Yeast Phenylalanyl tRNA

Solvent exchange rates of selected protons were measured by NMR saturation recovery method for yeast $tRNA^{Phe}$, at temperature from 25 to $40^{\circ}C$, in the presence of 0.1 M NaCl and various low levels of added magnesium ion. The exchange rates in zero $Mg^{2+}$ concentration indicate early m...

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Bibliographic Details
Published in:Bulletin of the Korean Chemical Society 1992, Vol.13 (5), p.517-520
Main Authors: Se Won Suh, Byong Seok Choi, Ki Hang Choi, Jin Young Park
Format: Article
Language:Korean
Online Access:Get full text
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Summary:Solvent exchange rates of selected protons were measured by NMR saturation recovery method for yeast $tRNA^{Phe}$, at temperature from 25 to $40^{\circ}C$, in the presence of 0.1 M NaCl and various low levels of added magnesium ion. The exchange rates in zero $Mg^{2+}$ concentration indicate early melting of acceptor stem, D stem, and tertiary structure. Addition of magnesium ion stabilizes the entire D stem more effectively than any other secondary or tertiary interactions.
ISSN:0253-2964
1229-5949