High-efficient Expression of Porcine IL-2 with Recombinant Baculovirus Infected Silkworm, Bombyx mori

Biologically active porcine Interleukin-2(poIL-2) was produced from in vitro and in viva baculovirus expression system, namely the Autographa californica nuclear polyhedrosis virus (ACNPV)-cell culture system and the Hybrid nucler polthedrosis virus (HyNPV)-sillkworm larva system. The concentration...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2000, Vol.5 (2), p.146-149
Main Authors: Inumaru, Shigeki, KokuHo, Takehiro, Yada, Takashi, Kiuchi, Makoto, Miyazawa, Mitsusuhiro
Format: Article
Language:Korean
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Summary:Biologically active porcine Interleukin-2(poIL-2) was produced from in vitro and in viva baculovirus expression system, namely the Autographa californica nuclear polyhedrosis virus (ACNPV)-cell culture system and the Hybrid nucler polthedrosis virus (HyNPV)-sillkworm larva system. The concentration of the recombinant poIL-2(rpoIL-2) in the larvae hemolymph was 1 to 3 mg/mL, which was about 7 to 20 times those of the cell culture systems. The level of this expression efficiency is equal to that with transgenic livestock, secretion products in milk.
ISSN:1226-8372
1976-3816