Preliminary Evidence for a Metallothionein-like Cd-binding Protein in the Kidney of the Antarctic Clam Laternula elliptica

A Cd-binding protein was identified in the renal cytosol of the Antarctic clam Laternula elliptica which naturally contains high concentrations of Cd. The Cd-binding protein showed similar characteristics of metallothionein (MT) in molecular weight (about 10-12 kDa) and low spectral absorbance at 28...

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Bibliographic Details
Published in:Ocean and polar research 2001, Vol.23 (4), p.337-345
Main Authors: Choi, Hee-Seon, J, Ahn In-Young, Ryu Soo-Kyung, Lee, Yong-Seok, Lee, In-Sook, Jeong, Kye-Heon
Format: Article
Language:Korean
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Summary:A Cd-binding protein was identified in the renal cytosol of the Antarctic clam Laternula elliptica which naturally contains high concentrations of Cd. The Cd-binding protein showed similar characteristics of metallothionein (MT) in molecular weight (about 10-12 kDa) and low spectral absorbance at 280 nm with relatively high absorbance at 254nm. Results of immuno-histochemical staining suggested that the MT-like Cd-binding protein was mainly located in the epithelial cells of the kidney. The MT-like protein was a major ligand of cytosolic Cd as shown in the elution profiles of chromatography and may play an important role in Cd sequestration and accumulation in L. elliptica kidney. A considerable amount of Cd was also found to be associated with particulate fraction, indicating the sequestration to particulate fraction is as important as binding to the cytosolic MT-like protein in Cd accumulation in the kidney.
ISSN:1598-141X
2234-7313