cDNA Cloning and mRNA Expression of A Cuticle Protein Gene Homo­logue from Protaetia brevitarsis

A cuticle protein gene, PbLCP12.1, from the white-spotted flower chafer, Protaetia brevitarsis, was isolated and characterized. The gene contains an ORF of 336 nucleotides capable of encoding a 113 amino acid polypeptide with a predicted molecular mass of 12,138 Da and pI of 4.15. The PbLCP12.1 prot...

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Bibliographic Details
Published in:International Journal of Industrial Entomology 2005-09, Vol.11 (1), p.67-70
Main Authors: Ik Soo Kim, Yong Soo Choi, Eun Mee Lee, Mi Ae Kim, Eun Young Yun, Mi Young Ahn, Byung Rae Jin
Format: Article
Language:Korean
Subjects:
cDNA cloning
Cuticle protein
Insect
Pro­taetia brevitarsis
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Summary:A cuticle protein gene, PbLCP12.1, from the white-spotted flower chafer, Protaetia brevitarsis, was isolated and characterized. The gene contains an ORF of 336 nucleotides capable of encoding a 113 amino acid polypeptide with a predicted molecular mass of 12,138 Da and pI of 4.15. The PbLCP12.1 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins. The deduced amino acid sequence of the PbLCP12.1 cDNA is most similar to Bombyx mori cuticle protein BmLCP18 (37% protein sequence identity). Northern blot analysis revealed that PbLCP12.1 showed the epidermis-specific expression.
ISSN:1598-3579