Optimization of ${\gamma}-Polyglutamic$ Acid Production by Bacillus subtilis ZJU-7 Using a Surface-response Methodology

The components of the media used to elicit the biosynthesis of $poly-{\gamma}-glutamic$ acid $({\gamma}-PGA)$ by Bacillus subtilis ZJU-7 were investigated, particularly the carbon and nitrogen sources Of the 7 carbon sources investigated, sucrose induced the highest rate of ${\gamma}-PGA$ productivi...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2006, Vol.11 (3), p.251-257
Main Authors: Shi, Feng, Xu, Zhinan, Cen, Peilin
Format: Article
Language:Korean
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Summary:The components of the media used to elicit the biosynthesis of $poly-{\gamma}-glutamic$ acid $({\gamma}-PGA)$ by Bacillus subtilis ZJU-7 were investigated, particularly the carbon and nitrogen sources Of the 7 carbon sources investigated, sucrose induced the highest rate of ${\gamma}-PGA$ productivity; among the nitrogen sources, tryptone had the best effect for ${\gamma}-PGA$ production. A $2^{6-2}$ fractional factorial design was used to screen factors that influence ${\gamma}-PGA$ production significantly, and a central composite design was finally adopted to formulate the optimal medium. ${\gamma}-PGA$ productivity improved approximately 2-fold when the optimal medium was used compared with the original nonoptimized medium, and volumetric productivity reached a maximum of 58.2 g/L after a 24-h cultivation period.
ISSN:1226-8372
1976-3816