Human brain pyridoxal-5`-phosphate phosphatase (PLPP) protein transduction of PEP-1-PLPP into PC12 cells

Pyridoxal-5'-phosphate phosphatase (PLPP) catalyzes the dephosphorylation of pyridoxal-5'-phosphate (PLP). A human brain PLPP gene was fused with a PEP-1 peptide and produced a genetic in-frame PEP-1-PLPP fusion protein. The purified PEP-1-PLPP fusion protein was efficiently transduced int...

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Bibliographic Details
Published in:BMB reports 2008-05, Vol.41 (5), p.408-413
Main Authors: Lee, Yeom-Pyo, Kim, Dae-Won, Lee, Min-Jung, Jeong, Min-Seop, Kim, So-Young, Lee, Sun-Hwa, Jang, Sang-Ho, Park, Jin-Seu, Kang, Tae-Cheon, Won, Moo-Ho, Cho, Sung-Woo, Kwon, Oh-Shin, Eum, Won-Sik, Choi, Soo-Young
Format: Article
Language:Korean
Subjects:
Human brain pyridoxal-5`-phosphate phosphatase
PEP-1 peptide
PLP
PLPP
Protein therapy
Protein transduction
Pyridoxal-5`-phosphate
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Summary:Pyridoxal-5'-phosphate phosphatase (PLPP) catalyzes the dephosphorylation of pyridoxal-5'-phosphate (PLP). A human brain PLPP gene was fused with a PEP-1 peptide and produced a genetic in-frame PEP-1-PLPP fusion protein. The purified PEP-1-PLPP fusion protein was efficiently transduced into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced PEP-1-PLPP fusion protein was stable for 36 h. The concentration of PLP was markedly decreased by the addition of exogenous PEP-1-PLPP to media pretreated with the vitamin $B_6$ precursors; pyridoxine, pyridoxal kinase and pyridoxine-5'-phosphate oxidase into cells. The results suggest that the transduction of the PEP-1-PLPP fusion protein can be one mode of PLP level regulation, and to replenish this enzyme in the various neurological disorders related to vitamin $B_6$.
ISSN:1976-6696
1976-670X