Functional Complementation of a Perilla ${\omega}-3$ Fatty Acid Desaturase under the Seed-specific SeFAD2 Promoter

Functional characterization of the fatty acid desaturase genes and seed-specific promoters is prerequisite for altering the unsaturated fatty acid content of oilseeds by genetic manipulation. The ${\omega}-6$ fatty acid desaturase (FAD2) and ${\omega}-3$ fatty acid desaturase (FAD3) catalyze extra-p...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2008, Vol.51 (3), p.174-179
Main Authors: Kim, Mi-Jung, Go, Young-Sam, Ahn, Sung-Ju, Chung, Chung-Han, Suh, Mi-Chung
Format: Article
Language:Korean
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Functional characterization of the fatty acid desaturase genes and seed-specific promoters is prerequisite for altering the unsaturated fatty acid content of oilseeds by genetic manipulation. The ${\omega}-6$ fatty acid desaturase (FAD2) and ${\omega}-3$ fatty acid desaturase (FAD3) catalyze extra-plastidial desaturation of oleic acid to linoleic acid and linoleic acid to linolenic acid, respectively. These are major constituents in seed storage oils. Here, we report the complementation of a perilla linoleic acid desaturase (PrFAD3) cDNA under the seed-specific sesame FAD2 (SeFAD2) promoter in the Arabidopsis fad3 mutant. PrFAD3 is functionally active and the SeFAD2 promoter is applicable for modifying fatty acid composition in developing seeds. Transient expression of the GUS gene under that promoter in the developing seeds and leaves of sesame, soybean, and corn via microprojectile bombardment indicated that the SeFAD2 promoter likely will be useful for altering the seed phenotypes of dicot and monocot crops.
ISSN:1226-9239
1867-0725