Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes αB-crystallin. Co-i...

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Bibliographic Details
Published in:BMB reports 2010-06, Vol.43 (6), p.432-437
Main Authors: Deng, Yun, Li, Yongqing, Fan, Xiongwei, Yuan, Wuzhou, Xie, Huaping, Mo, Xiaoyang, Yan, Yan, Zhou, Junmei, Wang, Yuequn, Ye, Xianli, Wan, Yongqi, Wu, Xiushan
Format: Article
Language:Korean
Subjects:
LBH
p21
p53
Yeast two-hybrid
αB-crystallin
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Summary:LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes αB-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with αB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, αB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or αB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both αB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and αB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21. [BMB reports 2010; 43(6): 432-437]
ISSN:1976-6696
1976-670X