Anthocyanins in Cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata]

The aim of this study was to isolate and identify the anthocyanins in the black seed coated cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata] using reverse phase C-18 open column chromatography and high performance liquid chromatography (HPLC) with diode array detection and electro spray ioniza...

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Bibliographic Details
Published in:Food science and biotechnology 2010, Vol.19 (3), p.821-826
Main Authors: Ha, Tae-Joung, Lee, Myoung-Hee, Jeong, Yu-Na, Lee, Jin-Hwan, Han, Sang-Ik, Park, Chang-Hwan, Pae, Suk-Bok, Hwang, Chung-Dong, Baek, In-Youl, Park, Keum-Yong
Format: Article
Language:Korean
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Summary:The aim of this study was to isolate and identify the anthocyanins in the black seed coated cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata] using reverse phase C-18 open column chromatography and high performance liquid chromatography (HPLC) with diode array detection and electro spray ionization/mass spectrometry (DAD-ESI/MS) analysis, respectively. Anthocyanins were extracted from the coat of black cowpea with 1% trifluoroacetic acid (TFA) in methanol, isolated by RP-C-18 column chromatography, and their structures elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. The isolated anthocyanins were characterized as delphinidin-3-O-glucoside (2) and cyanidin-3-Oglucoside (4). Furthermore, 5 minor anthocyanins were detected and identified as delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (5), peonidin-3-O-glucoside (7), and malvidin-3-O-glucoside (8) based on the fragmentation patterns of HPLC-DAD-ESI/MS analysis.
ISSN:1226-7708
2092-6456