Adipose-Derived Stem Cells Improve Efficacy of Melanocyte Transplantation in Animal Skin

• Published in: Biomolecules & therapeutics 2014-07, Vol.22 (4), p.328-333
• Main Authors: Lim, Won-Suk, Kim, Chang-Hyun, Kim, Ji-Young, Do, Byung-Rok, Kim, Eo Jin, Lee, Ai-Young
• Format: Article
• Language: Korean
• Subjects: Adipose-derived stem cells; Animal; Effi cacy; Grafting; Melanocytes; Short-term safety
• ISSN: 1976-9148; 2005-4483

Vitiligo is a pigmentary disorder induced by a loss of melanocytes. In addition to replacement of pure melanocytes, cocultures of melanocytes with keratinocytes have been used to improve the repigmentation outcome in vitiligo treatment. We previously identifi ed by in vitro studies, that adipose-derived stem cells (ADSCs) could be a potential substitute for keratinocytes in cocultures with melanocytes. In this study, the effi cacy of pigmentation including durability of grafted melanocytes and short-term safety was examined in the nude mouse and Sprague-Dawley rat after grafting of primary cultured human melanocytes, with or without different ratios of primary cultured human ADSCs. Simultaneous grafting of melanocytes and ADSCs, which were separately cultured and mixed on grafting at the ratios of 1:1, 1:2, or 1:3, showed better effi cacy than that of pure melanocytes. Grafting of melanocytes cocultured with ADSCs resulted in a similar outcome as the grafting of cell mixtures. Skin pigmentation by melanocytes : ADSCs at the ratios of 1:1 and 1:2 was better than at 1:3. No signifi cant difference was observed between the 1-week and 2-week durations in coculturing. Time-course microscopic examination showed that the grafted melanocytes remained a little longer than 6-week post-grafting. No infl ammatory cell infi ltration was observed in the grafted skin and no melanocytes were detectable in other organs. Collectively, grafting of melanocytes and ADSCs was equally safe and more effective than grafting of melanocytes alone. Despite the absence of signifi cant differences in effi cacy between the group of 1:1 and that of 1:2 ratio, 1:2 ratio for 1-week coculturing may be better for clinical use from the cost-benefi t viewpoint.