Physiological Response of Escherichia coli W3110 and BL21 to the Aerobic Expression of Vitreoscilla Hemoglobin

The aerobic growth and metabolic performance of Escherichia coli strains BL21 and W3110 were studied when the Vitreoscilla hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both strains and was nearly eliminated in BL21. Transcri...

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Bibliographic Details
Published in:Journal of microbiology and biotechnology 2020-10, Vol.30 (10), p.1592-1596
Main Authors: Lara, Alvaro R, Galindo, Janet, Jaen, Karim E, Juarez, Mariana, Sigala, Juan-Carlos
Format: Article
Language:Korean
Subjects:
aerobic cultures
BL21
coli W3110
E
transcriptional analysis
Vitreoscilla hemoglobin
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Summary:The aerobic growth and metabolic performance of Escherichia coli strains BL21 and W3110 were studied when the Vitreoscilla hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both strains and was nearly eliminated in BL21. Transcriptional levels of the glyoxylate shunt genes decreased in both strains when VHb was expressed. However, higher transcription of the α-ketoglutarate dehydrogenase genes were observed for W3110, while for BL21 transcription levels decreased. VHb expression reduced the transcription of the cytochrome bo 3 genes only in BL21. These results are useful for better selecting a production host.
ISSN:1017-7825
1738-8872