Expression and evaluation of porcine circovirus type 2 capsid protein mediated by recombinant adeno-associated virus 8

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Korea), 2021, Vol.22 (1), p.8.1-8.11
• Main Authors: Li, Shuang, Wang, Bo, Jiang, Shun, Lan, Xiaohui, Qiao, Yongbo, Nie, Jiaojiao, Yin, Yuhe, Shi, Yuhua, Kong, Wei, Shan, Yaming
• Format: Article
• Language: Korean
• ISSN: 1229-845X; 1976-555X

Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.