Loading…
Cordycepin from Medicinal Fungi Cordyceps militaris Mitigates Inflammaging-Associated Testicular Damage via Regulating NF-κB/MAPKs Signaling in Naturally Aged Rats
Inflammaging in male reproductive organs covers a wide variety of problems, including sexual dysfunction and infertility. In this study, the beneficial effects of cordycepin (COR), isolated from potential medicinal fungi Cordyceps militaris, in aging-associated testicular inflammation and serum bioc...
Saved in:
Published in: | Mycobiology 2022, Vol.50 (1), p.86-95 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | Korean |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | |
---|---|
cites | |
container_end_page | 95 |
container_issue | 1 |
container_start_page | 86 |
container_title | Mycobiology |
container_volume | 50 |
creator | Kopalli, Spandana Rajendra Cha, Kyu-Min Cho, Jae Youl Kim, Si-Kwan Koppula, Sushruta |
description | Inflammaging in male reproductive organs covers a wide variety of problems, including sexual dysfunction and infertility. In this study, the beneficial effects of cordycepin (COR), isolated from potential medicinal fungi Cordyceps militaris, in aging-associated testicular inflammation and serum biochemical changes in naturally aged rats were investigated. Male Sprague Dawley rats were divided into young control (YC), aged control (AC), and COR (5, 10, and 20 mg/kg) treated aged rat groups. Aging-associated serum biochemical changes and inflammatory parameters were analyzed by biochemical assay kits, Western blotting, and real-time RT-PCR. Results showed a significant (p < 0.05) alteration in the total blood cell count, lipid metabolism, and liver functional parameters in AC group when compared with YC group. However, COR-treated aged rats ameliorated the altered biochemical parameters significantly (p < 0.05 and p < 0.01 at 5, 10, and 20 mg/kg, respectively). Furthermore, the increase in the expression of inflammatory mediators (COX-2, interleukin (IL)-6, IL-1β, and tissue necrosis factor-alpha) in aged rat testis was significant (p < 0.05) when compared with YC group. Treatment with COR at 20 mg/kg to aged rats attenuated the increased expression of inflammatory mediators significantly (p < 0.05). Mechanistic studies revealed that the potential attenuating effects exhibited by COR in aged rats was mediated by regulation of NF-κB activation and MAPKs (c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38) signaling. In conclusion, COR restored the altered serum biochemical parameters in aged rats and ameliorated the aging-associated testicular inflammation proving the therapeutic benefits of COR targeting inflammaging-associated male sexual dysfunctions. |
format | article |
fullrecord | <record><control><sourceid>kisti</sourceid><recordid>TN_cdi_kisti_ndsl_JAKO202216458019810</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>JAKO202216458019810</sourcerecordid><originalsourceid>FETCH-kisti_ndsl_JAKO2022164580198103</originalsourceid><addsrcrecordid>eNqNjk1OwzAUhC0EEhH0Dm_DMsKx-5MsQyGCVi2odF89Esd6wnFQnoPU-3AKDsGZMBLsWY30zWhmTkSiZKHSQit9KpJMqSLNZaHPxYSZXuRM68U8ny4S8bHsh-ZYmzfy0A59BxvTUE0eHVSjtwR_PkNHjgIOxLChQBaDYXjwrcOuQ0vepiVzX1PkDewNB6pHhwPcYrQNvBPCztiIQszCtkq_Pm-uN-XTmuGZbBz8wfHFFsM4oHNHKG1s2mHgS3HWomMz-dULcVXd7Zf36SvFmYNv2B1W5fpRSaWy-XSWy6zIM6n_m_sGMX1eXA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Cordycepin from Medicinal Fungi Cordyceps militaris Mitigates Inflammaging-Associated Testicular Damage via Regulating NF-κB/MAPKs Signaling in Naturally Aged Rats</title><source>Taylor & Francis Open Access</source><source>PubMed</source><creator>Kopalli, Spandana Rajendra ; Cha, Kyu-Min ; Cho, Jae Youl ; Kim, Si-Kwan ; Koppula, Sushruta</creator><creatorcontrib>Kopalli, Spandana Rajendra ; Cha, Kyu-Min ; Cho, Jae Youl ; Kim, Si-Kwan ; Koppula, Sushruta</creatorcontrib><description>Inflammaging in male reproductive organs covers a wide variety of problems, including sexual dysfunction and infertility. In this study, the beneficial effects of cordycepin (COR), isolated from potential medicinal fungi Cordyceps militaris, in aging-associated testicular inflammation and serum biochemical changes in naturally aged rats were investigated. Male Sprague Dawley rats were divided into young control (YC), aged control (AC), and COR (5, 10, and 20 mg/kg) treated aged rat groups. Aging-associated serum biochemical changes and inflammatory parameters were analyzed by biochemical assay kits, Western blotting, and real-time RT-PCR. Results showed a significant (p < 0.05) alteration in the total blood cell count, lipid metabolism, and liver functional parameters in AC group when compared with YC group. However, COR-treated aged rats ameliorated the altered biochemical parameters significantly (p < 0.05 and p < 0.01 at 5, 10, and 20 mg/kg, respectively). Furthermore, the increase in the expression of inflammatory mediators (COX-2, interleukin (IL)-6, IL-1β, and tissue necrosis factor-alpha) in aged rat testis was significant (p < 0.05) when compared with YC group. Treatment with COR at 20 mg/kg to aged rats attenuated the increased expression of inflammatory mediators significantly (p < 0.05). Mechanistic studies revealed that the potential attenuating effects exhibited by COR in aged rats was mediated by regulation of NF-κB activation and MAPKs (c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38) signaling. In conclusion, COR restored the altered serum biochemical parameters in aged rats and ameliorated the aging-associated testicular inflammation proving the therapeutic benefits of COR targeting inflammaging-associated male sexual dysfunctions.</description><identifier>ISSN: 1229-8093</identifier><identifier>EISSN: 2092-9323</identifier><language>kor</language><ispartof>Mycobiology, 2022, Vol.50 (1), p.86-95</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,4024</link.rule.ids></links><search><creatorcontrib>Kopalli, Spandana Rajendra</creatorcontrib><creatorcontrib>Cha, Kyu-Min</creatorcontrib><creatorcontrib>Cho, Jae Youl</creatorcontrib><creatorcontrib>Kim, Si-Kwan</creatorcontrib><creatorcontrib>Koppula, Sushruta</creatorcontrib><title>Cordycepin from Medicinal Fungi Cordyceps militaris Mitigates Inflammaging-Associated Testicular Damage via Regulating NF-κB/MAPKs Signaling in Naturally Aged Rats</title><title>Mycobiology</title><addtitle>Mycobiology</addtitle><description>Inflammaging in male reproductive organs covers a wide variety of problems, including sexual dysfunction and infertility. In this study, the beneficial effects of cordycepin (COR), isolated from potential medicinal fungi Cordyceps militaris, in aging-associated testicular inflammation and serum biochemical changes in naturally aged rats were investigated. Male Sprague Dawley rats were divided into young control (YC), aged control (AC), and COR (5, 10, and 20 mg/kg) treated aged rat groups. Aging-associated serum biochemical changes and inflammatory parameters were analyzed by biochemical assay kits, Western blotting, and real-time RT-PCR. Results showed a significant (p < 0.05) alteration in the total blood cell count, lipid metabolism, and liver functional parameters in AC group when compared with YC group. However, COR-treated aged rats ameliorated the altered biochemical parameters significantly (p < 0.05 and p < 0.01 at 5, 10, and 20 mg/kg, respectively). Furthermore, the increase in the expression of inflammatory mediators (COX-2, interleukin (IL)-6, IL-1β, and tissue necrosis factor-alpha) in aged rat testis was significant (p < 0.05) when compared with YC group. Treatment with COR at 20 mg/kg to aged rats attenuated the increased expression of inflammatory mediators significantly (p < 0.05). Mechanistic studies revealed that the potential attenuating effects exhibited by COR in aged rats was mediated by regulation of NF-κB activation and MAPKs (c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38) signaling. In conclusion, COR restored the altered serum biochemical parameters in aged rats and ameliorated the aging-associated testicular inflammation proving the therapeutic benefits of COR targeting inflammaging-associated male sexual dysfunctions.</description><issn>1229-8093</issn><issn>2092-9323</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqNjk1OwzAUhC0EEhH0Dm_DMsKx-5MsQyGCVi2odF89Esd6wnFQnoPU-3AKDsGZMBLsWY30zWhmTkSiZKHSQit9KpJMqSLNZaHPxYSZXuRM68U8ny4S8bHsh-ZYmzfy0A59BxvTUE0eHVSjtwR_PkNHjgIOxLChQBaDYXjwrcOuQ0vepiVzX1PkDewNB6pHhwPcYrQNvBPCztiIQszCtkq_Pm-uN-XTmuGZbBz8wfHFFsM4oHNHKG1s2mHgS3HWomMz-dULcVXd7Zf36SvFmYNv2B1W5fpRSaWy-XSWy6zIM6n_m_sGMX1eXA</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Kopalli, Spandana Rajendra</creator><creator>Cha, Kyu-Min</creator><creator>Cho, Jae Youl</creator><creator>Kim, Si-Kwan</creator><creator>Koppula, Sushruta</creator><scope>JDI</scope></search><sort><creationdate>2022</creationdate><title>Cordycepin from Medicinal Fungi Cordyceps militaris Mitigates Inflammaging-Associated Testicular Damage via Regulating NF-κB/MAPKs Signaling in Naturally Aged Rats</title><author>Kopalli, Spandana Rajendra ; Cha, Kyu-Min ; Cho, Jae Youl ; Kim, Si-Kwan ; Koppula, Sushruta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kisti_ndsl_JAKO2022164580198103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kopalli, Spandana Rajendra</creatorcontrib><creatorcontrib>Cha, Kyu-Min</creatorcontrib><creatorcontrib>Cho, Jae Youl</creatorcontrib><creatorcontrib>Kim, Si-Kwan</creatorcontrib><creatorcontrib>Koppula, Sushruta</creatorcontrib><collection>KoreaScience</collection><jtitle>Mycobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kopalli, Spandana Rajendra</au><au>Cha, Kyu-Min</au><au>Cho, Jae Youl</au><au>Kim, Si-Kwan</au><au>Koppula, Sushruta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cordycepin from Medicinal Fungi Cordyceps militaris Mitigates Inflammaging-Associated Testicular Damage via Regulating NF-κB/MAPKs Signaling in Naturally Aged Rats</atitle><jtitle>Mycobiology</jtitle><addtitle>Mycobiology</addtitle><date>2022</date><risdate>2022</risdate><volume>50</volume><issue>1</issue><spage>86</spage><epage>95</epage><pages>86-95</pages><issn>1229-8093</issn><eissn>2092-9323</eissn><abstract>Inflammaging in male reproductive organs covers a wide variety of problems, including sexual dysfunction and infertility. In this study, the beneficial effects of cordycepin (COR), isolated from potential medicinal fungi Cordyceps militaris, in aging-associated testicular inflammation and serum biochemical changes in naturally aged rats were investigated. Male Sprague Dawley rats were divided into young control (YC), aged control (AC), and COR (5, 10, and 20 mg/kg) treated aged rat groups. Aging-associated serum biochemical changes and inflammatory parameters were analyzed by biochemical assay kits, Western blotting, and real-time RT-PCR. Results showed a significant (p < 0.05) alteration in the total blood cell count, lipid metabolism, and liver functional parameters in AC group when compared with YC group. However, COR-treated aged rats ameliorated the altered biochemical parameters significantly (p < 0.05 and p < 0.01 at 5, 10, and 20 mg/kg, respectively). Furthermore, the increase in the expression of inflammatory mediators (COX-2, interleukin (IL)-6, IL-1β, and tissue necrosis factor-alpha) in aged rat testis was significant (p < 0.05) when compared with YC group. Treatment with COR at 20 mg/kg to aged rats attenuated the increased expression of inflammatory mediators significantly (p < 0.05). Mechanistic studies revealed that the potential attenuating effects exhibited by COR in aged rats was mediated by regulation of NF-κB activation and MAPKs (c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38) signaling. In conclusion, COR restored the altered serum biochemical parameters in aged rats and ameliorated the aging-associated testicular inflammation proving the therapeutic benefits of COR targeting inflammaging-associated male sexual dysfunctions.</abstract><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1229-8093 |
ispartof | Mycobiology, 2022, Vol.50 (1), p.86-95 |
issn | 1229-8093 2092-9323 |
language | kor |
recordid | cdi_kisti_ndsl_JAKO202216458019810 |
source | Taylor & Francis Open Access; PubMed |
title | Cordycepin from Medicinal Fungi Cordyceps militaris Mitigates Inflammaging-Associated Testicular Damage via Regulating NF-κB/MAPKs Signaling in Naturally Aged Rats |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T00%3A32%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-kisti&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cordycepin%20from%20Medicinal%20Fungi%20Cordyceps%20militaris%20Mitigates%20Inflammaging-Associated%20Testicular%20Damage%20via%20Regulating%20NF-%CE%BAB/MAPKs%20Signaling%20in%20Naturally%20Aged%20Rats&rft.jtitle=Mycobiology&rft.au=Kopalli,%20Spandana%20Rajendra&rft.date=2022&rft.volume=50&rft.issue=1&rft.spage=86&rft.epage=95&rft.pages=86-95&rft.issn=1229-8093&rft.eissn=2092-9323&rft_id=info:doi/&rft_dat=%3Ckisti%3EJAKO202216458019810%3C/kisti%3E%3Cgrp_id%3Ecdi_FETCH-kisti_ndsl_JAKO2022164580198103%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |