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Prolonged semen incubation alters the biological characteristics of human spermatozoa

Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37℃. Methods: Twenty-five normozoospermic semen samples were incubated at 37℃. Incubation was performed at four time intervals of 0 (afte...

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Bibliographic Details
Published in:Clinical and experimental reproductive medicine 2022, Vol.49 (4), p.270-276
Main Authors: Sayed Abbas Datli Beigi, Mohammad Ali Khalili, Ali Nabi, Mohammad Hosseini, Abolghasem Abbasi Sarcheshmeh, Mojdeh Sabour
Format: Article
Language:Korean
Online Access:Get full text
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Summary:Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37℃. Methods: Twenty-five normozoospermic semen samples were incubated at 37℃. Incubation was performed at four time intervals of 0 (after liquefaction), 1, 1.5, and 2 hours. The samples were evaluated for sperm parameters at each time interval. Results: The rate of sperm progressive motility decreased at 1.5 hours compared to 0 hours as well as 2 hours compared to 1 hour and 0 hours. The rate of non-motile spermatozoa also decreased after 2 hours compared to after 0 hours. No significant changes were observed in sperm viability (p=0.98) and non- progressive motility (p=0.48) at any time intervals. Abnormal sperm morphology increased at 1.5 hours of incubation time (p
ISSN:2233-8233
2233-8241