Human sperm parameter improvement associated with Ceratonia siliqua extract as a cryopreservation supplement after vitrification

• Published in: Clinical and experimental reproductive medicine 2023, Vol.50 (2), p.86-93
• Main Authors: Tooba Farazmand, Fatemeh Mansouri, Yeganeh Koohestanidehaghi, Erfan Shahandeh
• Format: Article
• Language: Korean
• ISSN: 2233-8233; 2233-8241

Objective: Given the destructive effects of oxidative stress on sperm structure, this study was conducted to investigate the antioxidant effects of different concentrations of Ceratonia siliqua plant extract on human sperm parameters after the freezing-thawing process. Methods: A total of 20 normozoospermic samples were frozen. Each sample was divided into two control groups (fresh and cryopreservation) and three cryopreservation experimental groups (containing C. siliqua extract at concentrations of 20, 30, and 40 ㎍/mL in the freezing extender). Motility, intracellular levels of reactive oxygen species (ROS), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), viability, and acrosome reaction parameters were evaluated. Results: Statistical analysis showed that the highest motility, viability, and PMI were associated with the 20 ㎍/mL concentration of C. siliqua extract. At all concentrations, intracellular ROS levels were significantly lower and the levels of MMP and the acrosome reaction were significantly higher than in the cryopreservation control group (p≤0.05). Conclusion: C. siliqua extract supplements at concentrations of 20, 30, and 40 ㎍/mL improved sperm motility, viability, PMI, MMP, intracellular ROS, and the acrosome reaction.