Common light signaling pathways controlling DNA repair and circadian clock entrainment in zebrafish

UV radiation causes a number of harmful events including growth delay, cell death and ultimately cancer. The reversal of such effects by concomitant exposure to visible light is a conserved mechanism which has been uncovered in many multi-cellular organisms. Here we show that light-dependent UV-tole...

Full description

Saved in:
Bibliographic Details
Published in:Cell cycle (Georgetown, Tex.) Tex.), 2009-09, Vol.8 (17), p.2794-2801
Main Authors: Hirayama, Jun, Miyamura, Norio, Uchida, Yoshimi, Asaoka, Yoichi, Honda, Reiko, Sawanobori, Kenji, Todo, Takeshi, Yamamoto, Takuro, Sassone-Corsi, Paolo, Nishina, Hiroshi
Format: Article
Language:English
Subjects:
Animals
Binding
Biological Clocks - physiology
Biology
Bioscience
Calcium
Cancer
Cell
Cells, Cultured
Circadian Rhythm - physiology
Cryptochromes - metabolism
Cycle
Deoxyribodipyrimidine Photo-Lyase - metabolism
DNA Repair
Extracellular Signal-Regulated MAP Kinases - metabolism
Eye Proteins - metabolism
Landes
Light Signal Transduction
MAP Kinase Signaling System
Organogenesis
p38 Mitogen-Activated Protein Kinases - metabolism
Period Circadian Proteins - metabolism
Phosphorylation
Proteins
Proto-Oncogene Proteins c-myc - metabolism
Time Factors
Ultraviolet Rays
Zebrafish - metabolism
Zebrafish Proteins - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:UV radiation causes a number of harmful events including growth delay, cell death and ultimately cancer. The reversal of such effects by concomitant exposure to visible light is a conserved mechanism which has been uncovered in many multi-cellular organisms. Here we show that light-dependent UV-tolerance is a cell autonomous phenomenon in zebrafish. In addition, we provide several lines of evidence indicating that light induction of 64PHR, a DNA repair enzyme, and the subsequent light-dependent DNA repair mediated by this enzyme are prerequisites for light-mediated UV tolerance. 64PHR is evolutionary related to and has a high degree of structural similarity to animal CRY, an essential circadian regulator. The zebrafish circadian clock is controlled by a cell-autonomous and light-dependent oscillator, where zCRY1a functions as an important mediator of light entrainment of the circadian clock. In this study, we show that light directly activates MAPK signaling cascades in zebrafish cells and we provide evidence that light-induced activation of these pathways controls the expression of two evolutionary-related genes, z64Phr and zCry1a, revealing that light-dependent DNA repair and the entrainment of circadian clock share common regulatory pathways.
ISSN:1538-4101
1551-4005
DOI:10.4161/cc.8.17.9447