Differential expression of dnaA and dosR genes among members of the Mycobacterium tuberculosis complex under oxic and hypoxic conditions

Major differences regarding the pathology and host immune response of the Beijing and Canettii genotypes of Mycobacterium tuberculosis have been reported; however, studies on the genetic expression of these genotypes during in vitro dormancy are scarce. This study examined the expression of five cel...

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Bibliographic Details
Published in:International microbiology 2010-03, Vol.13 (1), p.9-13
Main Authors: Badillo-López, Claudia, González-Mejía, Arturo, Helguera-Repetto, A Cecilia, Salas-Rangel, Laura P, Rivera-Gutiérrez, Sandra, Cerna-Cortés, Jorge F, González-Y-Merchand, José A
Format: Article
Language:English
Subjects:
Antigens, Bacterial - biosynthesis
Antigens, Bacterial - genetics
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Humans
Hypoxia
Mycobacterium tuberculosis - growth & development
Mycobacterium tuberculosis - metabolism
Mycobacterium tuberculosis - physiology
Oxygen - metabolism
Protein Kinases - biosynthesis
Protein Kinases - genetics
RNA, Messenger - biosynthesis
RNA, Messenger - metabolism
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Summary:Major differences regarding the pathology and host immune response of the Beijing and Canettii genotypes of Mycobacterium tuberculosis have been reported; however, studies on the genetic expression of these genotypes during in vitro dormancy are scarce. This study examined the expression of five cell-cycle-related genes and two dormancy-related genes in M. canettii, M. tuberculosis H37Rv, and M. tuberculosis Beijing during the Wayne model of dormancy. The results showed that under hypoxic conditions the three tuberculosis genotypes were able to transcribe genes involved in DNA replication and cellular division. In addition, dosR was found to be up-regulated in M. tuberculosis Beijing during the exponential growth phase but down-regulated under hypoxic conditions. In this genotype, the replication-related gene dnaA was also strongly down-regulated. These latter two findings suggest that, compared to M. tuberculosis H37Rv and M. canettii, the Beijing genotype has a lower capacity to synthesize dosR, hspX, and dnaA mRNAs during in vitro dormancy.
ISSN:1139-6709
1618-1905
DOI:10.2436/20.1501.01.106