Transmembrane transport of fluoride studied by time-resolved emission spectroscopy

Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(III) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of t...

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Bibliographic Details
Main Authors: Alessio Cataldo, Matúš Chvojka, Gyeongjin Park, Vladimír Šindelář, François P Gabbaï, Stephen Butler, Hennie Valkenier
Format: Article
Language:English
Subjects:
Chemical sciences
Engineering
Organic Chemistry
Online Access:Request full text
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Summary:Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(III) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of the assay allowed us to study different types of fluoride transporters and unravel their mechanisms of action.