Extracellular ATP-evoked norepinephrine release coupled with receptor-operated Ca permeable channels in PC12 cells

Characteristics of extracellular ATP-stimulated norepinephrine (NE) secretion were investigated by the batch method [J. Biol. Chem. ,263, 8157, 1988], the whole cell voltage-clamp technique [Pflugers Arch., 408, 112, 1987], and fura-2 method. ATP stimulated intracellular Ca increase and ^^3 H-NE sec...

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Published in:Japanese Journal of Pharmacology 1990, Vol.52 (suppl-1.1), p.100-100
Main Authors: Kazuhide Inoue, Ken Nakazawa, Kannosuke Fujimori, Tomoko Obama, Isao Saito, Akira Takanaka
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container_title Japanese Journal of Pharmacology
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Ken Nakazawa
Kannosuke Fujimori
Tomoko Obama
Isao Saito
Akira Takanaka
description Characteristics of extracellular ATP-stimulated norepinephrine (NE) secretion were investigated by the batch method [J. Biol. Chem. ,263, 8157, 1988], the whole cell voltage-clamp technique [Pflugers Arch., 408, 112, 1987], and fura-2 method. ATP stimulated intracellular Ca increase and ^^3 H-NE secretion from PC12 cells in the concentration range from 15 μM to 1 mM. ATP also activated an inward current in a similar dose-dependent manner to the secretion, and depolarized cell membrane. The secretion and increase of intracellular Ca were extracellular Ca-dependent, but were not inhibited by Ca-channel blockers, nicardipine (up to 10μM) or cadmium (up to 300μM). The relative potencies of analogues in evoking secretion were in the same order as the activating currents (ATP > ATP-g-S ≫ ADP). AMP or adenosine had no effect. These findings suggest that ATP-stimulated NE secretion is coupled with the ATP receptor-activated Ca permeable channel in PC12 cells.
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title Extracellular ATP-evoked norepinephrine release coupled with receptor-operated Ca permeable channels in PC12 cells
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