Diazepam inhibits Ba-currents in PC12 cells

The effects of diazepam (DZP) on voltage-gated Ca channels were studied in PC12 cells using whole-cell voltage-clamp techniques. The voltage-step-evoked Ba-currents were completely blocked by a low concentration of cadmium (Cd, 30 μM) and were also sensitive to nicardipine (100 nM to 10 μM). DZP (1-...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1992, Vol.58 (suppl.2), p.379-379
Main Authors: Kazuhide Inoue, Ken Nakazawa, Kannosuke Fujimori, Norio Akaike, Akira Takanaka
Format: Article
Language:Japanese
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Summary:The effects of diazepam (DZP) on voltage-gated Ca channels were studied in PC12 cells using whole-cell voltage-clamp techniques. The voltage-step-evoked Ba-currents were completely blocked by a low concentration of cadmium (Cd, 30 μM) and were also sensitive to nicardipine (100 nM to 10 μM). DZP (1-100 μM) inhibited the Ba currents in a concentration-dependent manner. Neither DZP nor nicardipine affected the current-voltage relationship or the dependence on holding potentials of the Ba currents. When DZP was applied to the cells in combination with nicardipine, the observed inhibition agreed with a value predicted assuming independent blockade by DZP and by nicardipine. These results suggest that DZP inhibits Ca channels in a manner similar to nicardipine, but that binding sites for DZP are different from those for nicardipine.
ISSN:0021-5198