Removal of Extracellular Mg^2+ Suppresses Sulfation of Glycoconjugates Secreted from Rabbit Trachea in Culture

The influences of extracellular Ca^2+ and Mg^2+ concentrations on the basal secretion of glycoconjugates from rabbit trachea in organ culture were examined. Over 80% of the ^^35 S-labeled and [^^3 H]glucosamine-labeled glycoconjugates secreted by the trachea were digested upon incubation with chondr...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1992, Vol.60 (3), p.247-252
Main Authors: Hirofumi Kai, Aishi Kimoto, Kazuhisa Yoshitake, Sachiko Matsumoto, Yoichiro Isohama, Kazuo Takahama, Takeshi Miyata
Format: Article
Language:Japanese
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Summary:The influences of extracellular Ca^2+ and Mg^2+ concentrations on the basal secretion of glycoconjugates from rabbit trachea in organ culture were examined. Over 80% of the ^^35 S-labeled and [^^3 H]glucosamine-labeled glycoconjugates secreted by the trachea were digested upon incubation with chondroitinase ABC. The basal secretion did not occur in the medium at 4℃, indicating an energydependent process. The basal secretion at 37℃ of ^^35 S-labeled glycoconjugates was prominently suppressed in Mg^2+ -free Tyrode solution but not in Ca^2+ -free Tyrode solution containing ethyleneglycol bis(2-aminoethylether)tetraacetic acid (EGTA). In contrast, the basal secretion of [^^3 H]glucosamine-labeled glycoconjugates was not affected by the Mg^2+ concentration in the medium. The results suggest that extracellular Mg^2+ largely contributes to sulfation of glycoconjugates basally secreted from rabbit trachea.
ISSN:0021-5198