α - And p-adrenocepter-mediated cAMP formation in cerebral cortex of spontaneously hypertensive rat

Spontaneously hypertensive rat (SHR) has been reported to show some abnormal responses to stimulation of monoamine receptors. In this study, we investigated α- and β-adrenocepter-mediated cAMP accumulation in SHR and Wistar Kyoto rat (WKY) brain. Cerebral cortical slices of 6 week-old SHR and WKY we...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1993, Vol.61 (suppl.1), p.248-248
Main Authors: Yukako Yamazaki, Yuji Kiuchi, Masahiro Inagaki, Noriyuki Nakachi, Tomoyoshi Uchikawa, Chie Yokomizo, Katsuji Oguchi
Format: Article
Language:Japanese
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Summary:Spontaneously hypertensive rat (SHR) has been reported to show some abnormal responses to stimulation of monoamine receptors. In this study, we investigated α- and β-adrenocepter-mediated cAMP accumulation in SHR and Wistar Kyoto rat (WKY) brain. Cerebral cortical slices of 6 week-old SHR and WKY were incubated at 37℃ for 10 min with isoproterenol (ISO) or norepinephrine (NE) in Krebs-Ringer buffer containing 0.5 mM IBMX. α/β-agonist NE(100 μM) increased cAMP accumulation by 4.5 times of basal level in both SHR and WKY. On the other hand, β-agonist ISO (1 μM) increased cAMP accumulation by 4 times in SHR slices and by 2 times in WKY slices. Our results show that response to β-adrenocepter stimulation is more pronounced in SHR than WKY brain, and that synergistic interaction of α-adrenoceptor with β-adrenoceptor-mediated response is not obvious in SHR, suggesting functional uncoupling between α-and β-adrenoceptors.
ISSN:0021-5198