Signal transduction of serotonin 1C receptor in cDNA-transfected cells

We investigated serotonin 1C receptor (5-HT1CR) signal transduction using Xenopus oocytes injected with mRNA, and found that 5-HT1CR coupled with phospholipase C, low molecular G proteins were involved in the 5-HT1CR response, with some of antidepressants blocking the 5-HT1CR response as antagonists...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1993, Vol.61 (suppl.2), p.330-330
Main Authors: Chihiro Kumagai, Haruko Oda, Junji Nakamura, Michihisa Tohda, Yasuyuki Nomura
Format: Article
Language:Japanese
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Summary:We investigated serotonin 1C receptor (5-HT1CR) signal transduction using Xenopus oocytes injected with mRNA, and found that 5-HT1CR coupled with phospholipase C, low molecular G proteins were involved in the 5-HT1CR response, with some of antidepressants blocking the 5-HT1CR response as antagonists, and so on. However, direct evidence has not shown which 5-HT1CR enhances production of inositol phosphates (IPs). To further clarify 5-HT1CR signal transduction and cellular function, we transfected 5-HT1CR cDNA to COS-7 cells and examined IPs formation evoked by 5-HT stimulation. The entire rat 5-HT1CR coding sequence was inserted in expression vector pKCRH possessing SV40 early promoter, and the constructed vector was transfected into COS-7 cells by the DEAE-dextran method. Application of 5-HT to the transfected cells increased production of inositol phosphates in a time-dependent manner. Pretreatment with 10 μM mianserin or 10 μM imipramine for 5 min blocked 5-HT-induced IPs foFmation. We are further investigating effects of other antidepressants on 5-HT1CR-induced IPs formation and mechanism of low molecular G proteins involved in the pathway bringing about PI turnover.
ISSN:0021-5198