Novel diterpenoids, scabronine A and G, secrete neurotrophic factors from 1321N1 human astrocytoma cells

Glial cells release neurotrophic factors which are essential to maintain neurons functionally. When rat pheochromocytoma cells (PC-12) were cultivated in the human astrocytoma cell (1321N1) culture media conditioned by new diterpenoids, scabronine A and G, isolated from Sarcodon scabrosus, the cells...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1999, Vol.79 (suppl.1), p.106-106
Main Authors: Yutaro Obara, Norimichi Nakahata, Takako Kita, Yoshiaki Takaya, Tomihisa Ohta, Yoshiteru Oshima, Yasushi Ohizumi
Format: Article
Language:Japanese
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Summary:Glial cells release neurotrophic factors which are essential to maintain neurons functionally. When rat pheochromocytoma cells (PC-12) were cultivated in the human astrocytoma cell (1321N1) culture media conditioned by new diterpenoids, scabronine A and G, isolated from Sarcodon scabrosus, the cells extremely changed their morphology with neurite outgrowth. These diterpenoids induced nerve growth factor (NGF) secretion from 1321N1 cells in a concentration-dependent manner. Furthermore, they increased NGF mRNA expression in 1321N1 cells. However, the enhancement of the differentiation of PC-12 cells by the conditioned media was only slightly inhibited by the treatment with neutralizing antibody against NGF. And the concentration of the released NGF by scabronines was bellow to cause the differentiation, suggesting that scabronines cause the differentiation of PC-12 cells by secretion of NGF and additional factors from 1321N1 cells. These diterpenoids can keep neuronal function through a secretion from neurotrophic factors from glial cells, and are useful to clarify the mechanism of synthesis and secretion of neurotrophic factors.
ISSN:0021-5198