The mechanism of catecholamine secretion induced by PACAP in the perfused rat adrenal gland

We investigated the mechanism of catecholamine (CA) secretion induced by pituitary adenylate cyclase-activating polypeptide (PACAP) in the isolated perfused rat adrenal gland. PACAP (10-100 nM) caused concentration-dependent increases in CA output from adrenal gland. PACAP-(6-38) (30-3000nM), a PACA...

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Published in:Japanese Journal of Pharmacology 2000, Vol.82 (suppl.1), p.126-126
Main Authors: Yasuo Fukushima, Hisako Kawashima, Takahiro Nagayama, Hiroaki Hisa, Tomohiko Kimura, Susumu Satoh
Format: Article
Language:Japanese
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Summary:We investigated the mechanism of catecholamine (CA) secretion induced by pituitary adenylate cyclase-activating polypeptide (PACAP) in the isolated perfused rat adrenal gland. PACAP (10-100 nM) caused concentration-dependent increases in CA output from adrenal gland. PACAP-(6-38) (30-3000nM), a PACAP receptor blocker, inhibited increases in CA output induced by PACAP. [Lys^1 ,Pro^2,5 ,AGR^3,4 ,Tyr^6 ]-VIP (LPAT-VIP) (30-300 nM), a VIP receptor blocker, did not affect the PACAP-induced increases in CA output. PACAP-(6-38), but not LPAT-VIP, also inhibited increases in CA output induced by transmural electrical stimulation. MDL-12,330A (10-100 μM), an adenylate cyclase inhibitor, inhibited increase in epinephrine output induced by PACAP without affecting the PACAP-induced increase in norepinephrine output. These results suggest that PACAP causes CA secretion via PACAP receptors and endogenous PACAP modulates neuronal control of CA secretion. Moreover, it is suggested that the mechanism of PACAP-induced epinephrine secretion, but not norepinephrine secretion, involves activation of adenylate cyclase.
ISSN:0021-5198