O-30155 Molecular cloning and analysis of a receptor for lysophosphatidylcholine in rat endothelial cells

Lysophosphatidylcholine (LPC), produced by the action of phospholipase A2 on phosphatidylcholine, is a component of oxidized low-density lipoprotein, and plays a proinflammatory and proatherogenic role in endothelial cells. Recently, human and mouse G2A, an orphan C protein-coupled receptor, has bee...

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Published in:Journal of Pharmacological Sciences 2004, Vol.94 (suppl.1), p.112-112
Main Authors: Kazuhiko Inoue, Shin-ichi Iwata, Takao Shimizu, Atsuro Miyata
Format: Article
Language:Japanese
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Summary:Lysophosphatidylcholine (LPC), produced by the action of phospholipase A2 on phosphatidylcholine, is a component of oxidized low-density lipoprotein, and plays a proinflammatory and proatherogenic role in endothelial cells. Recently, human and mouse G2A, an orphan C protein-coupled receptor, has been identified as a high-affinity receptor for LPC, and its expression was observed in lymphocytes and macrophages but not in endothelial cells. On the other hand, the structure and tissue distribution of rat LPC receptor have not been reported so far. These findings attempted us to isolate the endothelial LPC receptor in the rat. We performed RT-PCR with primers based on conservative sequence between mouse and human G2A using rat aortic endothelial cells mRNA. Unexpectedly, the cloned cDNA was revealed to be rat G2A, of which sequence exhibited 72 % and 87 % amino acid sequence identity with its human and mouse orthologs, respectively. We will also report its biochemical characteristics, intracellular signaling cascades, and the mechanisms of LPC-G2A interaction in endothelial cells.
ISSN:1347-8613