Expression and characterization of a novel antidepressant related gene#102 in rat brain

We have performed EST analysis to identify some molecular machinery responsible for antidepressant effects (ADRG#1-707). In the present study, we found the expression of ADRG#102 was significantly decreased by antidepressant treatment and electroconvulsive treatment (ECT), another effective treatmen...

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Bibliographic Details
Published in:Journal of Pharmacological Sciences 2004, Vol.94 (suppl.3), p.234-234
Main Authors: Natsuko Tsukahara, Misa Yamada, Kou Takahashi, Teruhiko Higuchi, Mitsuhiko Yamada, Kazutaka Momose
Format: Article
Language:Japanese
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Summary:We have performed EST analysis to identify some molecular machinery responsible for antidepressant effects (ADRG#1-707). In the present study, we found the expression of ADRG#102 was significantly decreased by antidepressant treatment and electroconvulsive treatment (ECT), another effective treatment for depression in rat frontal cortex using real time RT-PCR analysis. We determined the full length sequence of 4961 bp ADRG#l 02 encoded 265 amino acid residues containing four hydrophobic stretches of sufficient length to be membrane-spanning. Northern blot analysis in various tissue of adult rat revealed ADRG#102 mRNA was only detectable in rat brain. Interestingly, tissues from aging rat brain displayed the constant increase of the expression level was observed to 8weeks. Additionally, in rat cerebellar granul cell, ADRG#102 was localized in endoplasmic reticulum. Furthermore, ADRG#102 mRNA levels was increased in these cells after cell cultures. These results indicate that ADRG#102 may contribute to a novel molecular targets for the development of therapeutic agents.
ISSN:1347-8613