Mutagenic effects of UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts

Two types of DNA photolyase are known, CPD (cyclobutane pyrimidine dimer) photolyase and (6-4) photolyase. Both type of photolyase repair UV-induced DNA damage to normal dinucleotide using light energy. The repaired nucleoti does not show any mutagenecity. Using these DNA photolyase we are trying to...

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Bibliographic Details
Published in:JOURNAL OF RADIATION RESEARCH 1998, Vol.39 (4), p.364-364
Main Authors: Eriko OTOSHI, Takashi YAGI, Toshio MORI, Kenichi HITOMI, Tomoko ISHIKAWA, Mituo IKENAGA, Takeshi TODO
Format: Article
Language:Japanese
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Summary:Two types of DNA photolyase are known, CPD (cyclobutane pyrimidine dimer) photolyase and (6-4) photolyase. Both type of photolyase repair UV-induced DNA damage to normal dinucleotide using light energy. The repaired nucleoti does not show any mutagenecity. Using these DNA photolyase we are trying to compare the mutagenic effects of CPD and (6-4) photoproduct in human cell. UV-irradiated shuttle vector plasmids were treated with each or both type of DNA photolyase to remove either one of two type of UV lesion or both. The plasmid DNA were then transfected to XP-A cell and recovered from cells after culturing three days. Plasmids containing mutation on the SupF gene were selected on the plate containing X-gal and the induced mutation were determined by sequencing the SupF gene of the mutant plasmid. Both type of UV lesion was mutagenic.
ISSN:0449-3060