Isolation and characterization of Zebrafish (6-4)photolyase and CRY protein family

(6-4)photolyase family consists of two types of protein; one is (6-4)photolyase and another is blue light photoreceptor (CRY protein) which does not show any photorepair activity. The latter type of protein were found in mouse and human cells so far and its biological function has not yet been ident...

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Published in:JOURNAL OF RADIATION RESEARCH 1998, Vol.39 (4), p.366-366
Main Authors: Yuri KOBAYASHI, Tomoko ISHIKAWA, Tomohisa KATO Jr, Mituo IKENAGA, Takeshi TODO
Format: Article
Language:Japanese
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Summary:(6-4)photolyase family consists of two types of protein; one is (6-4)photolyase and another is blue light photoreceptor (CRY protein) which does not show any photorepair activity. The latter type of protein were found in mouse and human cells so far and its biological function has not yet been identified. To know the distribution of the (6-4)photolyase family among the present organism, we tried to isolate genes having homology to the (6-4)photolyase gene from Zebrafish. We have isolated three genes. One show most highest homology to the (6-4)photolyase gene isolated from Drosophila, Xenopus and Arabidopsis, and the recombinant protein expressed in E. coil show (6-4)photolyase acitivity, thus we classifid this clone as Z(6-4)photolyase. Another two show homology to the human/mouse Cry gene and does not show any photorepair activity when expressed in E coli, thus we classified these clones as ZCRYa and ZCryb gene.
ISSN:0449-3060