232 Synchrotron X-ray Microbeam Irradiation System. 1. Outline of the System

In order to evaluate the risk of low dose radiation, a microbeam irradiation system has been developed, by which we can irradiate individually identified cells with monochromatic (5.35 keV) synchrotron X-ray microbeam. The system is composed of a focussing mirror system or an adjustable 4-D slit sys...

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Bibliographic Details
Published in:JOURNAL OF RADIATION RESEARCH 2003, Vol.44 (4), p.443-444
Main Authors: Katsumi KOBAYASHI, Noriko USAMI, Hiroshi MAEZAWA, Tohru HAYASHI, Kotaro HIEDA, Kaoru TAKAKURA
Format: Article
Language:Japanese
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Summary:In order to evaluate the risk of low dose radiation, a microbeam irradiation system has been developed, by which we can irradiate individually identified cells with monochromatic (5.35 keV) synchrotron X-ray microbeam. The system is composed of a focussing mirror system or an adjustable 4-D slit system to obtain a microbeam, a fluorescence microscopy (Olympus BX51WIS) equipped with a precise motor-controlled sample stage (Prior H101, stroke 100 mm × 75 mm, reproducibility of positioning 1 micron), and a cell image capture system (Hamamatsu ORCA-ER-1394). Beam size routinely available is 5 micron square or larger, depending upon the request. Beam intensity is about 6 Gy per min, independent of the beam size. Also we have developed a system software to control all the process with Visual Basic calling Image Pro plus as a subroutine. Presently, we can automat ically scan and identify the target in desired area of the dish and also irradiate them one by one automatically.
ISSN:0449-3060