EFFECTS OF DEHYDRATION AND/OR DROUGHT STRESS TO GENE EXPRESSION IN SYNECHOCYSTIS SP. PCC6803

To investigate a response of cyanobacteria, Synechocystis sp. PCC6803 to dehydration and drought, we made the equipment that maintains a stable condition in humidity and temperature. Cells grown in a liquid medium (BG11) were filtered on to a cellulose acetate membrane, and then were incubated under...

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Bibliographic Details
Published in:Journal of Plant Research 2001, Vol.114 (suppl), p.73-74
Main Authors: Masayuki Hirano, Katsuhiko Okada, Makiko Miyaji, Masahiko Ikeuchi, Mikio Tuzuki
Format: Article
Language:Japanese
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Summary:To investigate a response of cyanobacteria, Synechocystis sp. PCC6803 to dehydration and drought, we made the equipment that maintains a stable condition in humidity and temperature. Cells grown in a liquid medium (BG11) were filtered on to a cellulose acetate membrane, and then were incubated under 60 - 85% of relative humidity (RH) at 34 C. The water content that was calculated from their weight decreased to 20% in 2h and then was kept constant for 4h. The dehydrated cells kept under 85% RH for 2h (the water content is 20%) was all alive, but not any more after 4h. To elucidate open reading flames (ORFs) that respond to dehydration and/or drought, northern hybridization was performed. The ORF, sll0020 (clpC) which is homologous to erd1 (early response to dehydration 1) in high plants, was transcribed even in a liquid culture. The level of its transcript was increased in 30min to be the highest after lhr -treatment. We used DNA micro array to survey ORFs responding to dehydration and/or drought stress. The expression level was remarkably changed in about 3% of the total ORFs, when the cells were treated in 85% RH at 34℃ for lhr.
ISSN:0918-9440