Effects of (1R,9S)-β-Hydrastine on Intracellular Calcium Concentration in PC12 Cells

(1R,9S)-β-Hydrastine (BHS) decreases the basal intracellular Ca2+ concentration ([Ca2+]i) in PC12 cells.5) This study examined the effects of (1R,9S)-BHS on [Ca2+]i in PC12 cells. (1R,9S)-BHS at 10-100μm in combination with a high extracellular K+ level (56 mM) inhibited the release of dopamine in a...

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Published in:Biological & Pharmaceutical Bulletin 2007-08, Vol.30 (8), p.1547-1550
Main Authors: Shou Yu YINa, b, Yu Mi KIMa, Jae Joon LEEa, Chun Mei JINa, Yoo Jung YANGa, Seung-Kook PARKa, Se Kuel YOOc, Myung Koo LEEa
Format: Article
Language:Japanese
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Summary:(1R,9S)-β-Hydrastine (BHS) decreases the basal intracellular Ca2+ concentration ([Ca2+]i) in PC12 cells.5) This study examined the effects of (1R,9S)-BHS on [Ca2+]i in PC12 cells. (1R,9S)-BHS at 10-100μm in combination with a high extracellular K+ level (56 mM) inhibited the release of dopamine in a concentration-dependent manner with an IC50 value of 66.5μM. BHS at 100μM inhibited the sustained increase in [Ca2+]i induced by a high K+ level (56 mM), and had an inhibitory effect on the 2μm nifedipine-induced blockage of the K+-stimulated sustained increase in [Ca2+]i. In addition, (1R,9S)-BHS at 100μm prevented the rapid and sustained increase in [Ca2+]i elicited by 20 mM caffeine, but did not have an effect on the increase induced by 1μm thapsigargin, in the presence of external Ca2+. These results suggest that the active sites of (1R,9S)-BHS are mainly L-type Ca 2+ channels and caffeine-sensitive Ca 2+-permeable channels in PC12 cells.
ISSN:0918-6158