Evaluation of in Vitro Embryotoxicity of Dental Monomers by Differentiation of Embryonic Stem Cells into Contracting Cardiac Myocytes

The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3 cells)were examined using the embryonic stem cell test(EST). Three study parameters, the rate of differentiation into contracting cardiomyocytes, the 50% cell viability of ES-D3 and BAL...

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Bibliographic Details
Published in:Alternatives to Animal Testing and Experimentation 2007, Vol.12 (2), p.171-178
Main Authors: Koichi Imai, Toru Hayakawa, Masaaki Nakamura
Format: Article
Language:Japanese
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Summary:The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3 cells)were examined using the embryonic stem cell test(EST). Three study parameters, the rate of differentiation into contracting cardiomyocytes, the 50% cell viability of ES-D3 and BALB/c 3T3 cells, and the clone A31(3T3 cells), were used to determine the embryotoxicity Of the eighteen dental monomers tested, Bis-GMA(6F)and MTYA were classified as weakly embryotoxic, and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, 6-HHMA, MEPC, Phosmer M, BPE-1300, BSNa EDMABA, GAM, GMR, PTSNa and QTX were evaluated as nonembryotoxic. However, none of the monomers tested were found to be strongly embryotoxic. Our results suggest that funher intensive studies on the embryotoxicity of dental biomaterials are needed in addition to examination of conventional biological aspects from the perspective of biologica safety.
ISSN:1344-0411