Cell Viability Test for Alkalinized Lidocaine using the Three-dimensional Cultured Human Skin Model

「Abstract」Tissue damage by lidocaine and alkalinized lidocaine was studied using a three-dimensional cultured skin model. Water for injection, dibasic potassium hydrogen phosphate, and sodium bicarbonate were added to 4% lidocaine, and pH was measured. Dibasic potassium hydrogen phosphate and sodium...

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Bibliographic Details
Published in:Alternatives to Animal Testing and Experimentation 2008, Vol.13 (3), p.123-126
Main Authors: Yoshihiro Momota, Koichi Imai, Naotaka Kishimoto, Kazutomo Yamabayashi, Junichiro Kotani
Format: Article
Language:Japanese
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Summary:「Abstract」Tissue damage by lidocaine and alkalinized lidocaine was studied using a three-dimensional cultured skin model. Water for injection, dibasic potassium hydrogen phosphate, and sodium bicarbonate were added to 4% lidocaine, and pH was measured. Dibasic potassium hydrogen phosphate and sodium bicarbonate were added to phosphate buffered saline (PBS) (pH 7.4) and adjusted so that they would be the same as the obtained pH (7.85, 7.90). Each of the test drugs were added to TESTSKIN(TM) (LSE-d) and cultured, and were compared using the MTT method regarding cell viability. The formazan absorbance decreased in the alkalinized lidocaine compared to PBS and alkalinized PBS. On the other hand, no significant difference was observed in formazan absorbance between lidocaine that was not alkalinized (pH 6.7) and PBS, so it is believed that the alkalinization of lidocaine may have a possibility of decreasing cell viability.
ISSN:1344-0411