14. Human placenta feeder layers support undifferentiated growth of primate embryonic stem cells

Undifferentiated various embryonic stem (ES) cells can grow on mouse embryonic fibroblast (MEF) feeders. The risk of animal pathogens cross-transfering from animal feeds to human ES cells can be excluded using human feeder layers. Here we show that human placenta can be a useful feeder layer for the...

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Bibliographic Details
Published in:Journal of Hard Tissue Biology 2003, Vol.12 (2), p.65-65
Main Authors: Kanji MIYAMOTO, Tomoaki YAMADA, Kazuhiko HAYASHI, Toshio YAMABE, Yoshihiro ITO
Format: Article
Language:Japanese
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Summary:Undifferentiated various embryonic stem (ES) cells can grow on mouse embryonic fibroblast (MEF) feeders. The risk of animal pathogens cross-transfering from animal feeds to human ES cells can be excluded using human feeder layers. Here we show that human placenta can be a useful feeder layer for the undifferentiated growth of primate ES cells. As on MEF feeder, primate ES cells cultured on human amniotic epithelial layers had an undifferentiated growth for 18 passages in culture. Cultured primate ES cells expressed the transcription factor Oct-4, a membrane alkaline phosphatase, and the stage-specific embryonic antigen (SSEA-4). Human placenta is quite novel and important because it would provide a relatively available source of feeders for growth of human ES cells for therapeutic purpose that is also free from ethicai complications.
ISSN:1341-7649