Geometry of artificial ECM Three-Dimensional Structure of Titanium-Web (TW) Promotes Differentiation of Human Bone Marrow Mesenchymal Cells into Osteoblasts

Abstract: We demonstrate that a new culture system using a titanium web (TW) consisted of titanium fibers (50 mm diameter) can promote differentiation of mesenchymal cells into osteoblasts without conventional application of dexamethasone. Human mesenchymal stem cells (hMSC, Cambrex) and MC3T3-E1 we...

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Published in:Journal of Hard Tissue Biology 2005, Vol.14 (2), p.333-334
Main Authors: Dong Li, Shouhei Iku, Kazuki Nemoto, Yasuo Kokai, Tetsuya Odajima, Ryota Yoshimoto, Tohru Kaku, Hideki Katoh, Hiroyuki Shiota, Yasuo Seki, Naomi Ogura, Yoshimitsu Abiko, Yoshinori Kuboki
Format: Article
Language:Japanese
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Summary:Abstract: We demonstrate that a new culture system using a titanium web (TW) consisted of titanium fibers (50 mm diameter) can promote differentiation of mesenchymal cells into osteoblasts without conventional application of dexamethasone. Human mesenchymal stem cells (hMSC, Cambrex) and MC3T3-E1 were cultured on titanium web (87% of porosity). ALP/DNA in hMSC at 2weeks was 2 times higher and MC3T3-E1 at 4 weeks was 4 times higher on TW than those on the conventional plastic dishes. Effect of 3D structure of TW is self-evident and the results will open a new age of 3D bone cell biology using TW. 「Introduction」There are increasing needs for efficient three-dimensional (3D) scaffold for cell culture systems, since it is becoming clear that there are remarkable differences in cellular behavior between the cells cultured on three-dimensional environment and cells cultured on the conventional flat dishes.1) But until recently only a few solid 3D biomaterials with sufficient porosity for cell growth have been provided.
ISSN:1341-7649