Protective Effects of Hydrogen-rich Water Intake on Renal Injury in Neonatal Rats with High Oxygen Loading

• Published in: Juntendo Medical Journal(2013-) 2022-06, Vol.68 (3), p.235-241
• Main Authors: MASAHITO SAITOH, AMANE ENDO, AKINA MATSUDA, HIROKI MIYANO, YUSUKE GONDA, AKIRA MIZUTANI, TAICHI HARA, MAYU NAKAGAWA, KOJI SAKURAYA, YAYOI MURANO, NAOTO NISHIZAKI, SHUICHIRO FUJINAGA, YOSHIYUKI OHTOMO, TOSHIAKI SHIMIZU
• Format: Article
• Language: Japanese
• ISSN: 2187-9737

Objectives: This study aimed to investigate the protective effects of hydrogen-rich water (HW) intake on renal injury in neonatal rats with high oxygen loading. Materials: We used pregnant and newborn Sprague-Dawley rats. Methods: Four groups were set up, with mother and newborn rats immediately after delivery as one group: RA-PW (room air and purified water), RA-HW (room air and HW), O2-PW (80% oxygen and purified water), and O2-HW (80% oxygen and HW). The newborn rats were maintained in either a normoxic (room air, 21% oxygen) or controlled hyperoxic (80% oxygen) environment from birth. Then, HW (O2-HW and RA-HW groups) or PW (O2-PW and RA-PW groups) was administered to parents of each group. Results: The number of immature glomeruli significantly increased in the O2-PW group (exposed to hyperoxia). Conversely, the O2-HW group had significantly fewer immature glomeruli than O2-PW group. In the RT-PCR analysis of kidney tissue, α-SMA, TGF-β, and TNF-α levels were significantly higher in the O2-PW group than in the RA-PW group and significantly lower in the O2-HW group than in the O2-PW group. Conclusions: HW intake can potentially reduce oxidative stress and prevent renal injury in neonates with high oxygen loading.