Mutagenicity of Arsenic in Mammalian Cells: Role of Reactive Oxygen Species

Arsenite, the trivalent form of arsenic present in the environment, is a known human carcinogen that lacked mutagenic activity in bacterial and standard mammalian cell mutation assays. We show herein that when evaluated in an assay (ALcell assay), in which both intragenic and multilocus mutations ar...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1998-07, Vol.95 (14), p.8103-8107
Main Authors: Hei, Tom K., Liu, Su X., Waldren, Charles
Format: Article
Language:English
Subjects:
Animals
Arsenic
Arsenic - toxicity
Arsenites
Biological Sciences
Cells
Cellular biology
CHO Cells
Chromosomes, Human, Pair 11
Cricetinae
Genes
Genetic mutation
Human chromosomes
Humans
Hybrid Cells
Life Sciences (General)
Mammals
Mutagenicity
Mutagenicity Tests
Mutagens
Mutation
Mutation - drug effects
Polymerase chain reaction
Reactive Oxygen Species
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cited_by cdi_FETCH-LOGICAL-c605t-ec7ff8ced504a073018723a93bbeb69bdf1cc989bcddde6b0e4e177a1d5214e63
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container_end_page 8107
container_issue 14
container_start_page 8103
container_title Proceedings of the National Academy of Sciences - PNAS
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creator Hei, Tom K.
Liu, Su X.
Waldren, Charles
description Arsenite, the trivalent form of arsenic present in the environment, is a known human carcinogen that lacked mutagenic activity in bacterial and standard mammalian cell mutation assays. We show herein that when evaluated in an assay (ALcell assay), in which both intragenic and multilocus mutations are detectable, that arsenite is in fact a strong dose-dependent mutagen and that it induces mostly large deletion mutations. Cotreatment of cells with the oxygen radical scavenger dimethyl sulfoxide significantly reduces the mutagenicity of arsenite. Thus, the carcinogenicity of arsenite can be explained at least in part by it being a mutagen that depends on reactive oxygen species for its activity.
doi_str_mv 10.1073/pnas.95.14.8103
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ispartof Proceedings of the National Academy of Sciences - PNAS, 1998-07, Vol.95 (14), p.8103-8107
issn 0027-8424
1091-6490
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source JSTOR Archival Journals and Primary Sources Collection【Remote access available】; PubMed Central
subjects Animals
Arsenic
Arsenic - toxicity
Arsenites
Biological Sciences
Cells
Cellular biology
CHO Cells
Chromosomes, Human, Pair 11
Cricetinae
Genes
Genetic mutation
Human chromosomes
Humans
Hybrid Cells
Life Sciences (General)
Mammals
Mutagenicity
Mutagenicity Tests
Mutagens
Mutation
Mutation - drug effects
Polymerase chain reaction
Reactive Oxygen Species
title Mutagenicity of Arsenic in Mammalian Cells: Role of Reactive Oxygen Species
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